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16s ribosomal rna gene pyrosequencing (v1-v3 region)  (Pyrosequencing Inc)

 
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    Structured Review

    Pyrosequencing Inc 16s ribosomal rna gene pyrosequencing (v1-v3 region)
    Characteristics of included studies
    16s Ribosomal Rna Gene Pyrosequencing (V1 V3 Region), supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/16s+ribosomal+rna+gene+pyrosequencing+(v1-v3+region)/16s+rrna+pyrosequencing/pmc07275316-29-89-93
    Average 90 stars, based on 1 article reviews
    16s ribosomal rna gene pyrosequencing (v1-v3 region) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "The gut microbiota profile of adults with kidney disease and kidney stones: a systematic review of the literature"

    Article Title: The gut microbiota profile of adults with kidney disease and kidney stones: a systematic review of the literature

    Journal: BMC Nephrology

    doi: 10.1186/s12882-020-01805-w

    Characteristics of included studies
    Figure Legend Snippet: Characteristics of included studies

    Techniques Used: Medications, Sequencing, Control, Amplification, Real-time Polymerase Chain Reaction, Generated, Microarray, Sterility, Sampling, Transplantation Assay

    Related Articles

    other:

    Article Title: Intestinal Microbiota Distinguish Gout Patients from Healthy Humans
    Article Snippet: All of the 83 samples were used for bacterial 16S rRNA genes V1-V3 region pyrosequencing and 39 samples (include 17 gout patients, 18 control individuals and 5 test individuals) were selected for whole-genome shotgun (WGS) sequencing.

    Article Title: Polycyclic Aromatic Hydrocarbon-Induced Changes in Bacterial Community Structure under Anoxic Nitrate Reducing Conditions
    Article Snippet: After 6 months enrichment with two culture transfers (See Materials and Methods Section), samples were taken to analyze changes in the bacterial communities using 16S rRNA gene V1–V3 region pyrosequencing.

    Article Title: Differential fecal microbiota are retained in broiler chicken lines divergently selected for fatness traits
    Article Snippet: All of the 29 samples were used for bacterial 16S rRNA genes V1-V3 region pyrosequencing and for whole-genome shotgun (WGS) sequencing.

    Article Title: Naphthalene biodegradation under oxygen‐limiting conditions: community dynamics and the relevance of biofilm‐forming capacity
    Article Snippet: We isolated the total DNA of duplicate biofilm samples taken at the oil–water interface to determine the structure of the community present using 16S rRNA gene V1–V3 region pyrosequencing.

    Article Title: Bacterial Communities Associated with Atherosclerotic Plaques from Russian Individuals with Atherosclerosis
    Article Snippet: The bacterial community composition in atherosclerotic plaques derived from common carotid arteries of twenty eight individual patients with clinical atherosclerosis was analyzed by V1–V3 16S rRNA gene region pyrosequencing analysis.

    Bacteria:

    Article Title: From Chihuahua to Saint-Bernard: how did digestion and microbiota evolve with dog sizes
    Article Snippet: .. Helicobacter spp are presents and could be pathogens Duodenum et Jejunum: Predominance of Eubacterium, Bacteroides, Clostridium, Fusobacterium, Bifidobacterium and Lactobacillus spp Ileum: Bacteroides, Eubacterium, Fusobacterium, Clostridium, Lactobacillus and Bifidobacterium predominate among the anaerobe bacteria There is also aerobe and facultative anaerobe bacteria: Streptococcus, Staphylococcus, Pasteurella, Escherichia and Enterobacter High density and diversity, Predominant bacteria are from Bacteroides, Bifidobacterium, Fusobacterium, Peptostreptococcus, Eubacterium, Clostridium, Peptococcus and Lactobacillus N/A Handl et al., 2011 12 N/A 0.7- 10.2 10 breeds N/A N/A N/A N/A 16S rRNA gene pyrosequencing (V1-V3 region) N/A N/A N/A Actinobacteria: 1.8% Bacteroidetes: 2.2% Firmicutes: 95% Fusobacteria: 0.3% Suchodolski, 2011 N/A N/A N/A N/A N/A N/A N/A N/A Review (or unpublished data) N/A 16S rRNA gene analysis: 2 archaeal phyla: Crenarchaeota et Euryarchaeota. .. Most abundant class: Methanobacteria (Methanosphaera et Methanobrevibacter) N/A 454 pyrosequencing: 4 fungal phyla with 90% Ascomycota > 5% Neocallimastigomycota Metagenomic sequencing: Archaea represents 1.1% of total microbiota (methanogens are the most abundant) Viruses are mainly bacteriophages and dsDNA viruses represent 0.4% of detected sequences Jia et al., 2010 8 N/A 4-13 N/A Commercial diet 18.2 9.9 CF: 1.6 SF: 5.8 IF: 1.2 FISH DGGE N/A N/A N/A Firmicutes: 31% Actinobacteria: 12% Bacteroidetes: <1% (108 cells/g feces) References N= Body weight (kg) Age (years) Breed Food Methods Gut microbiota composition Type of food Protein (%) Fat (%) Fiber (%) Stomach Small intestine Large intestine Stools Suchodolski et al., 2009 N/A N/A N/A N/A N/A N/A N/A 16S rRNA pyrosequencing N/A Jejunum mucosa: Proteobacteria: 46.7% Firmicutes: 15% Actinobacteria: 11.2% Spirochaetes: 14.2% Bacteroidetes: 6.2% Fusobacteria: 5.4% Tenericutes, Cyanobacteria, Verrucomicrobia and Chloroflexi: < 0.1% Gammaproteobacteria: 40% Spirochaetes: 13% Actinobacteria: 12% Clostridia: 10% Bacteroides: 6% Fusobacteria: 4% Alphaproteobacteria: 3% N/A N/A Biagi, Cipollini, & Zaghini, 2008 8 N/A N/A N/A N/A N/A N/A N/A Plate count N/A N/A N/A (Log CFU/mL) Lactobacilli: 7.9 Coliforms: 7.7 Enterococci: 7.5 Suchodolski et al., 2008b 64 N/A N/A N/A N/A N/A N/A N/A Illumina 16S rRNA gene sequencing N/A Fungi DNA detected in 60.9% of dogs and significatively increased in mucosal samples (82.8%) compared to luminal samples (42.9%): mainly Ascomycota and Basidiomycota.

    Medications:

    Article Title: The gut microbiota profile of adults with kidney disease and kidney stones: a systematic review of the literature
    Article Snippet: .. Shi 2014 [ ] , China , N = 52 n = 22 HD group Dialysis vintage: 6-40 months, Kt/V not reported. n = 30 reported ESKD not undertaking dialysis. eGFR, age, gender and ethnicity not reported. , N = 10 eGFR, age, gender and ethnicity not reported. , Details of sample collection not reported; Samples immersed in 90% alcohol and stored at -20°C , Excluded if taken in prior 3 weeks. , Medications not reported; Corticosteroids, statins, cytotoxic drugs not taken in prior 3 weeks. , SILVA , 16S ribosomal RNA gene Pyrosequencing (V1-V3 region) Sequencing platform not reported , ↑ α-diversity in HD group vs. controls (average OTUs/ species, p =0.044) and Chao1 index higher in three HD samples ( p -value not reported). β-diversity not reported between study groups. However, PCA plot of Unifrac data from selected samples at the genus level exhibited a large separation in the same three HD samples vs. the other selected samples ( p -value or statistical test not report). , Not reported. .. Stadlbauer 2017 [ ] , Austria , N = 30 n = 15 PD group GFR (ml/min): 7.9 (7.3; 14.0); Dialysis vintage: 25 (15-74) months. Age: 62yrs (54-69), 80% male. n = 15 HD group GFR (ml/min): 6.0 (5.9; 9.3); Dialysis vintage: 70 (40-197) months, Kt/V not reported. Age: 61yrs (54-71), 67% male; Ethnicity not reported. , N = 21 GFR (ml/min): 77.6 (73.4; 86.6). Age: 58 yrs (53-62), 43% male. Ethnicity not reported. , Self-sampled; Stored at -80°C , Not reported , Participants from HD and PD groups treated with phosphate binders, PPI, immunosuppressive drugs. Small number of controls ( n =2) also treated with PPI. , SILVA , 16S ribosomal RNA gene sequencing (V1-V2 region) Illumina MiSeq , ↓ α-diversity in PD and HD group compared to controls (Chao1 index, p <0.05, similar results reported for observed species/OTUs and phylogenic diversity) β-diversity significantly different between HD vs. controls p =0.012 and PD vs. controls p =0.003 (ANOSIM test based on Bray Curtis, weighted and unweighted UniFrac metrics) , Not reported.

    Sequencing:

    Article Title: The gut microbiota profile of adults with kidney disease and kidney stones: a systematic review of the literature
    Article Snippet: .. Shi 2014 [ ] , China , N = 52 n = 22 HD group Dialysis vintage: 6-40 months, Kt/V not reported. n = 30 reported ESKD not undertaking dialysis. eGFR, age, gender and ethnicity not reported. , N = 10 eGFR, age, gender and ethnicity not reported. , Details of sample collection not reported; Samples immersed in 90% alcohol and stored at -20°C , Excluded if taken in prior 3 weeks. , Medications not reported; Corticosteroids, statins, cytotoxic drugs not taken in prior 3 weeks. , SILVA , 16S ribosomal RNA gene Pyrosequencing (V1-V3 region) Sequencing platform not reported , ↑ α-diversity in HD group vs. controls (average OTUs/ species, p =0.044) and Chao1 index higher in three HD samples ( p -value not reported). β-diversity not reported between study groups. However, PCA plot of Unifrac data from selected samples at the genus level exhibited a large separation in the same three HD samples vs. the other selected samples ( p -value or statistical test not report). , Not reported. .. Stadlbauer 2017 [ ] , Austria , N = 30 n = 15 PD group GFR (ml/min): 7.9 (7.3; 14.0); Dialysis vintage: 25 (15-74) months. Age: 62yrs (54-69), 80% male. n = 15 HD group GFR (ml/min): 6.0 (5.9; 9.3); Dialysis vintage: 70 (40-197) months, Kt/V not reported. Age: 61yrs (54-71), 67% male; Ethnicity not reported. , N = 21 GFR (ml/min): 77.6 (73.4; 86.6). Age: 58 yrs (53-62), 43% male. Ethnicity not reported. , Self-sampled; Stored at -80°C , Not reported , Participants from HD and PD groups treated with phosphate binders, PPI, immunosuppressive drugs. Small number of controls ( n =2) also treated with PPI. , SILVA , 16S ribosomal RNA gene sequencing (V1-V2 region) Illumina MiSeq , ↓ α-diversity in PD and HD group compared to controls (Chao1 index, p <0.05, similar results reported for observed species/OTUs and phylogenic diversity) β-diversity significantly different between HD vs. controls p =0.012 and PD vs. controls p =0.003 (ANOSIM test based on Bray Curtis, weighted and unweighted UniFrac metrics) , Not reported.



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    Pyrosequencing Inc 16s ribosomal rna gene pyrosequencing (v1-v3 region)
    Characteristics of included studies
    16s Ribosomal Rna Gene Pyrosequencing (V1 V3 Region), supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/16s+ribosomal+rna+gene+pyrosequencing+(v1-v3+region)/16s+rrna+pyrosequencing/pmc07275316-29-89-93
    Average 90 stars, based on 1 article reviews
    16s ribosomal rna gene pyrosequencing (v1-v3 region) - by Bioz Stars, 2026-09
    90/100 stars
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    Characteristics of included studies

    Journal: BMC Nephrology

    Article Title: The gut microbiota profile of adults with kidney disease and kidney stones: a systematic review of the literature

    doi: 10.1186/s12882-020-01805-w

    Figure Lengend Snippet: Characteristics of included studies

    Article Snippet: Shi 2014 [ ] , China , N = 52 n = 22 HD group Dialysis vintage: 6-40 months, Kt/V not reported. n = 30 reported ESKD not undertaking dialysis. eGFR, age, gender and ethnicity not reported. , N = 10 eGFR, age, gender and ethnicity not reported. , Details of sample collection not reported; Samples immersed in 90% alcohol and stored at -20°C , Excluded if taken in prior 3 weeks. , Medications not reported; Corticosteroids, statins, cytotoxic drugs not taken in prior 3 weeks. , SILVA , 16S ribosomal RNA gene Pyrosequencing (V1-V3 region) Sequencing platform not reported , ↑ α-diversity in HD group vs. controls (average OTUs/ species, p =0.044) and Chao1 index higher in three HD samples ( p -value not reported). β-diversity not reported between study groups. However, PCA plot of Unifrac data from selected samples at the genus level exhibited a large separation in the same three HD samples vs. the other selected samples ( p -value or statistical test not report). , Not reported.

    Techniques: Medications, Sequencing, Control, Amplification, Real-time Polymerase Chain Reaction, Generated, Microarray, Sterility, Sampling, Transplantation Assay